Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Extracellular accumulation of amyloid-beta (Aβ) plaques is one the major pathological hallmarks Alzheimer's disease (AD), and target only FDA-approved disease-modifying treatment for AD. Accordingly, use transgenic mouse models that overexpress amyloid precursor protein thereby accumulate cerebral Aβ are widely used to model human AD in mice. Therefore, immunoassays, including enzyme-linked immunosorbent assay (ELISA) immunostaining, commonly measure load brain tissues derived from Though methods detection quantification have been well established documented, impact size region interest selected tissue on measurements following immunostaining has not reported. current protocol aimed compare across full- sub-regions using an image analysis software. The steps involved preparation, free-floating section imaging, versus described sections 13-month-old APP/PS1 double male results provide valuable information about Aβ-positive area quantification, show a strong correlation between obtained analyses mice widespread deposition.